2017-12-15 16:53:552025-05-24 13:27:06

description

transcriptional pleiotropic regulator (MerR family) involved in global nitrogen regulation

transcriptional pleiotropic regulator ([SW|MerR family]) involved in global nitrogen regulation

locus

BSU13310

BSU_13310

geneLength

330

333

product

transcription activator/ repressor (MerR family)

transcription activator/ repressor ([SW|MerR family])

outlinks

bsu

BSU13310

BSU_13310

Gene

Coordinates

1,397,411 → 1,397,743

1,397,411 1,397,743

Biological materials

Mutant

GP252 (in frame deletion), available in the [SW|Stülke] lab

BKE13310 (''tnrA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

GP2262 (''tnrA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)

1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]

BKE13310 (Δ[[gene|tnrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT

BKK13310 (Δ[[gene|tnrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT

GP252 (in frame deletion), available in [SW|Jörg Stülke]'s lab

BKE13310 (''tnrA''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]

GP2262 (''tnrA''::''ermC'') (available in [SW|Jörg Stülke]'s lab)

1A851 ( ''tnrA''::''erm''), [Pubmed|8799114], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A851&Search=1A851 BGSC]

BKE13310 ([[gene|tnrA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT

BKK13310 ([[gene|tnrA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTTCCACCCCTGGATG, downstream forward: _UP4_TAAATAATAAAAGTCCGGCT

Biological materials

Expression vector

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP171 available in [SW|Stülke] lab

pGP229 (N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]), available in [SW|Stülke] lab

Labs working on this gene/protein

[SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

[SW|Maria Schumacher], Durham, NC, USA [http://www.dukeschumacherlab.com/index.html homepage]

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|HTH merR-type domain] (aa 13-81) (according to UniProt)

The protein

additional information

information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000026 PRODORIC2 database]

Biological materials

Expression vectors

for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP171 available in [SW|Jörg Stülke]'s lab

pGP229 (N-terminal Strep-tag, for [SW|SPINE], expression in ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

labs

[SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

[SW|Maria Schumacher], Durham, NC, USA [http://www.dukeschumacherlab.com/index.html homepage]